The Influence of the Formulation Factors on Physico-chemical Properties of Dextran Associated Gantrez an Nanoparticles

Gantrez AN (a copolymer between methyl vinyl ether and maleic anhydride) nanoparticles were prepared by the solvent displacement method, and different dextrans with different molecular weight were associated to these nanoparticles in order to modify their surface characteristics. The resulting nanoparticles were characterized in terms of size, zeta potential, amount of dextran associated and dextran association efficiency. Using multifactorial analysis, we have studied the influence of formulation factors on nanoparticles’ characteristics mentioned above. The formulation factors studied were the following: the type of dextran used, the preparation method, the incubation time of dextran with the polymer and the quantity of dextran used. The molecular weight of dextran used was the major factor that influenced all nanoparticle properties evaluated, while the other factors studied had limited influence on nanoparticles’ characteristics. Rezumat S-au preparat nanoparticule din Gantrez AN (copolimer al metilvinil eterului cu anhidrida maleică) prin metoda deplasării solventului. S-a asociat dextran cu mase moleculare diferite acestor nanoparticule pentru modificarea proprietăţilor lor de suprafaţă. Nanoparticulele obţinute au fost caracterizate din punct de vedere al mărimii, potenţialului zeta, cantităţii de dextran asociate şi a eficienţei asocierii. Utilizând analiza multifactorială, am studiat efectul factorilor de formulare asupra caracteristicilor nanoparticulelor menţionate anterior. Factorii de formulare studiaţi au fost tipul de dextran utilizat, metoda de preparare, timpul de incubare al polimerului cu dextranul şi cantitatea de dextran utilizată. Masa moleculară a dextranului a fost cel mai important factor care a influenţat toate proprietăţile nanoparticulelor care au fost evaluate, în timp ce ceilalţi factori de formulare au avut o influenţă limitata asupra caracteristicilor nanoparticulelor.